Journal: Frontiers in Pharmacology

Article Title: Eplerenone Attenuates Fibrosis in the Contralateral Kidney of UUO Rats by Preventing Macrophage-to-Myofibroblast Transition

doi: 10.3389/fphar.2021.620433

Figure Lengend Snippet: Effects of long term-UUO and treatment of eplerenone (EPL) on renal histology, fibrosis, and function of the contralateral kidneys. Kidney sections from all groups were stained with HE for morphology changing (A) , Masson's Trichrome (Masson) for fibrosis (B) , and Sirius red for collagen deposition (C) . Images are representative for n = 12 rats each group. (D) BUN and Scr in serum were evaluated for renal function. Renal scores and quantification data are presented as mean ± SEM, n = 6. * p < 0.05 vs. the sham, # p < 0.05 vs. the UUO.

Article Snippet: After surgeries, eplerenone (Pfizer, United States) was given to UUO + Epl group via diet at the dose of 1.25 g/kg diet (equal to 100 mg/kg/day) for 6 months and other groups of rats were fed on regular chow.

Techniques: Staining

Journal: Frontiers in Pharmacology

Article Title: Eplerenone Attenuates Fibrosis in the Contralateral Kidney of UUO Rats by Preventing Macrophage-to-Myofibroblast Transition

doi: 10.3389/fphar.2021.620433

Figure Lengend Snippet: Effects of long term-UUO and treatment of eplerenone (EPL) on the expression of MR downstream molecules. Western blot for protein level expression of SGK1, (p)SGK1, NGAL and MCP-1 in the contralateral kidneys of rats treated with Sham, UUO and UUO + eplerenone (EPL). Data are mean ± SEM for n = 12 rats each group. * p < 0.05 vs. the sham, # p < 0.05 vs. the UUO.

Article Snippet: After surgeries, eplerenone (Pfizer, United States) was given to UUO + Epl group via diet at the dose of 1.25 g/kg diet (equal to 100 mg/kg/day) for 6 months and other groups of rats were fed on regular chow.

Techniques: Expressing, Western Blot

Journal: ACS chemical neuroscience

Article Title: Passage Variation of PC12 Cells Results in Inconsistent Susceptibility to Externally Induced Apoptosis

doi: 10.1021/acschemneuro.6b00208

Figure Lengend Snippet: Optimization and validation of etoposide-induced cell death in PC12 cells (passage 9–13). 50 × 103 cells/well. (a) Approximately 50% cell death was observed after 48 h. Four replicates per experiment. Values are represented as mean ± SD. Multiple t test, Holm-Sidak method, alpha = 5.000%, ****p < 0.0001. (b) Fluorescence measured after 48 h. Flupirtine at 3 μM concentration rescued the cells from apoptosis. Experiment repeated thrice. Values are represented as mean ± SD. Unpaired two-tailed t test, 95% CI, ****p < 0.0001 in comparison to vehicle. ####p < 0.0001 in comparison to etoposide treatment.

Article Snippet: 5 Etoposide (Chem-Impex International, 28435) was stored as a working stock solution of 150 μ g/mL at −20 °C for up to 3 months.

Techniques: Fluorescence, Concentration Assay, Two Tailed Test, Comparison

Journal: ACS chemical neuroscience

Article Title: Passage Variation of PC12 Cells Results in Inconsistent Susceptibility to Externally Induced Apoptosis

doi: 10.1021/acschemneuro.6b00208

Figure Lengend Snippet: NGF differentiated PC12 cells show a statistically significant difference in sensitivity to apoptosis induced by etoposide between early and late passages at 72 h post insult. (a) Passage 10. Three replicates per experiment. Values are represented as mean ± SD. One-way ANOVA, Dunnett test, 95% CI. ****p < 0.0001. (b) Passage 17. Three replicates per experiment. Values are represented as mean ± SD. One-way ANOVA, Dunnett test, 95% CI. ****p < 0.0001. (c) Comparison of 72 h etoposide treatment in passage 10 and 17 PC12 cells differentiated using NGF. Unpaired two-tailed t test, 95% CI, ****p < 0.0001.

Article Snippet: 5 Etoposide (Chem-Impex International, 28435) was stored as a working stock solution of 150 μ g/mL at −20 °C for up to 3 months.

Techniques: Comparison, Two Tailed Test